Comparing the use of chloroform to petroleum ether for Soxhlet extraction of fat in meat

Context Soxhlet extraction is commonly used for determining fat content of meat, because it the basis AOAC Final Action method this analysis. Petroleum ether as an solvent but other solvents, such chloroform, can be used. Chloroform has been stated to a safer than petroleum and extensively meat research in Australia; yet, there no report that validates its use purpose.Aim To compare chloroform with beef.Methods The six beef samples, reported chemical lean (CL, 100 – %fat), was determined using (by two independent providers) (b.p. 40–60°C, three commercial providers). Passing–Bablok regression Bland–Altman plot analysis were identify any differences bias between methods respectively.Key results showed difference beef. indicated evident uses each solvent. Thus, would indicate extraction, providing equivalency ether. While some minor apparent, most likely attributable sample heterogeneity.Conclusions found provide from meat. apparent attributed heterogeneity.Implications